Hotspot

accD

LSC · 55045-56907 · 1863 bp

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Pi 0.0382

MarkerSeek score 70.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1863
variable_sites: 98
pi: 0.0382
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAGAAAGGTTCT	CGGAAAGACTAGGCCCACTA	50.0	55.0	58.0	58.5	2502-2627 bp	0.666667	69.8
accD_p2	ATAGGGGGCAGAAAGGTTCT	TCCTTCCGAGAAATCTGGGA	50.0	50.0	58.0	57.8	2290 bp	0.333333	51.2
accD_p3	TCCTTGGGAATGGTGGATCT	TCCTTCCGAGAAATCTGGGA	50.0	50.0	58.0	57.8	2748 bp	0.333333	51.2
accD_p4	ATAGGGGGCAGAAAGGTTCT	TGGTTAAAGTCGCGAACTCA	50.0	45.0	58.0	57.5	2154 bp	0.333333	49.1
accD_p5	TCCTTGGGAATGGTGGATCT	TGGTTAAAGTCGCGAACTCA	50.0	45.0	58.0	57.5	2612 bp	0.333333	49.1