Hotspot

ndhI-ndhA

SSC · 116463-116564 · 102 bp

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Pi 0.0458

MarkerSeek score 79.40

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 7
pi: 0.0458
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TCCAATGTACCTTGCAGCTC	AGATGGACTTTACCTAGGATGAGA	50.0	41.7	57.9	58.0	291 bp	1.0	48.8
ndhI-ndhA_p2	GTCCAATGTACCTTGCAGCT	AGATGGACTTTACCTAGGATGAGA	50.0	41.7	58.2	58.0	292 bp	1.0	48.3
ndhI-ndhA_p3	TCCAATGTACCTTGCAGCTC	TGACAACTTCTTCTCAACTTGT	50.0	36.4	57.9	55.8	203 bp	1.0	48.3
ndhI-ndhA_p4	ACCTTGCAGCTCGTATTGTT	AGATGGACTTTACCTAGGATGAGA	45.0	41.7	57.8	58.0	283 bp	1.0	48.2
ndhI-ndhA_p5	TCCAATGTACCTTGCAGCTC	TGGACTTTACCTAGGATGAGA	50.0	42.9	57.9	54.8	288 bp	1.0	47.7