Hotspot

ccsA-ndhD

SSC · 112362-112628 · 267 bp

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Pi 0.0886

MarkerSeek score 72.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 267
variable_sites: 35
pi: 0.0886
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTTTACCCAAGGTTTCGCCA	TTTTTGATTCTGGGCCACGA	45.0	45.0	57.9	58.0	1059-1061 bp	1.0	84.9
ccsA-ndhD_p2	AATGCATCAACCCGCAGTAT	TTTTTGATTCTGGGCCACGA	45.0	45.0	57.9	58.0	1020 bp	0.333333	52.8
ccsA-ndhD_p3	ATGCATCAACCCGCAGTATT	TTTTTGATTCTGGGCCACGA	45.0	45.0	57.9	58.0	1019 bp	0.333333	52.8
ccsA-ndhD_p4	AATGCATCAACCCGCAGTAT	TTCTCGATGGCTTCTCTTGC	45.0	50.0	57.9	58.0	1240 bp	0.333333	52.8
ccsA-ndhD_p5	ATGCATCAACCCGCAGTATT	TTCTCGATGGCTTCTCTTGC	45.0	50.0	57.9	58.0	1239 bp	0.333333	52.8