Hotspot

clpP-psbB

LSC · 69345-69874 · 530 bp

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Pi 0.0627

MarkerSeek score 81.30

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 530
variable_sites: 48
pi: 0.0627
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTTTTGGAACACCAATTGGCA	GGCCCAACCAGAAACTAGAG	42.9	55.0	57.8	57.9	653-741 bp	1.0	76.7
clpP-psbB_p2	ACTTTTGGAACACCAATTGGC	GGCCCAACCAGAAACTAGAG	42.9	55.0	57.8	57.9	654-742 bp	1.0	76.7
clpP-psbB_p3	CTTTTGGAACACCAATTGGCA	CGATACCAAGGCAAACCCAT	42.9	50.0	57.8	58.2	571-659 bp	1.0	76.7
clpP-psbB_p4	CTTTTGGAACACCAATTGGCA	TGGACAGAAAGCAATCGACC	42.9	50.0	57.8	58.2	619-707 bp	1.0	76.5
clpP-psbB_p5	ACTTTTGGAACACCAATTGGC	TGGACAGAAAGCAATCGACC	42.9	50.0	57.8	58.2	620-708 bp	1.0	76.4