Hotspot

petA-psbJ

LSC · 60824-61758 · 935 bp

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Pi 0.0504

MarkerSeek score 80.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 67
pi: 0.0504
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	GAGAACCGACAAGGGATTCC	50.0	55.0	58.0	58.0	1529-1612 bp	1.0	87.8
petA-psbJ_p2	TGCATCAGATGGACGTCAAG	GAGAACCGACAAGGGATTCC	50.0	55.0	58.0	58.0	1715-1798 bp	1.0	87.3
petA-psbJ_p3	GGCATCTGTGATTTTGGCAC	TTTGTCTCTAGCATGACCGC	50.0	50.0	58.0	58.0	1182-1185 bp	0.666667	68.8
petA-psbJ_p4	TGCATCAGATGGACGTCAAG	TTTGTCTCTAGCATGACCGC	50.0	50.0	58.0	58.0	1368-1371 bp	0.666667	68.4
petA-psbJ_p5	GCATCAGATGGACGTCAAGT	TTTGTCTCTAGCATGACCGC	50.0	50.0	58.0	58.0	1367-1370 bp	0.666667	68.4