Hotspot

trnF-GAA-ndhJ

LSC · 46154-46704 · 551 bp

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Pi 0.0459

MarkerSeek score 78.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 551
variable_sites: 35
pi: 0.0459
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ACGCCCAATTTCTATGAAATTCA	50.0	34.8	58.1	57.1	497-641 bp	1.0	57.1
trnF-GAA-ndhJ_p2	TACTGGTCGGGATAGCTCAG	ACGCCCAATTTCTATGAAATTCA	55.0	34.8	58.0	57.1	541-685 bp	1.0	57.0
trnF-GAA-ndhJ_p3	GTACTGGTCGGGATAGCTCA	ACGCCCAATTTCTATGAAATTCA	55.0	34.8	58.3	57.1	542-686 bp	1.0	54.6
trnF-GAA-ndhJ_p4	TACTGGTCGGGATAGCTCAG	ACGCCCAATTTCTATGAAATTCAA	55.0	33.3	58.0	57.7	541-685 bp	1.0	54.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ACGCCCAATTTCTATGAAATTCAA	50.0	33.3	58.1	57.7	497-641 bp	1.0	54.1