Hotspot

trnL-UAA-trnF-GAA

LSC · 45776-46080 · 305 bp

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Pi 0.0699
MarkerSeek score 78.00
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
305
variable_sites
30
pi
0.0699
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 TTCAAGTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 50.0 50.0 57.0 58.1 384-511 bp 1.0 81.5
trnL-UAA-trnF-GAA_p2 TTCAAGTCCCTCTATCCCCA TACCAACTGAGCTATCCCGA 50.0 50.0 57.0 57.6 346-473 bp 1.0 78.8
trnL-UAA-trnF-GAA_p3 GGGTTCAAGTCCCTCTATCC TTTGAACTGGTGACACGAGG 55.0 50.0 56.7 58.1 387-514 bp 1.0 78.7
trnL-UAA-trnF-GAA_p4 GGTTCAAGTCCCTCTATCCC TTTGAACTGGTGACACGAGG 55.0 50.0 56.7 58.1 386-513 bp 1.0 78.7
trnL-UAA-trnF-GAA_p5 GTTCAAGTCCCTCTATCCCC TTTGAACTGGTGACACGAGG 55.0 50.0 56.7 58.1 385-512 bp 1.0 78.7