Hotspot

trnS-GCU-trnG-GCC

LSC · 7914-8320 · 407 bp

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Pi 0.059

MarkerSeek score 79.30

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 36
pi: 0.059
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	476-552 bp	1.0	59.0
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	475-551 bp	1.0	59.0
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	478-554 bp	1.0	57.9
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	477-553 bp	1.0	57.9
trnS-GCU-trnG-GCC_p5	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	479-555 bp	1.0	57.8