Hotspot

psbI-trnS-GCU

LSC · 7693-7825 · 133 bp

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Pi 0.0909

MarkerSeek score 78.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 18
pi: 0.0909
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	773-821 bp	1.0	89.0
psbI-trnS-GCU_p2	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	774-822 bp	1.0	89.0
psbI-trnS-GCU_p3	TTTTCTTCGCCAAATTGCCC	TGGACTAAAGCGTCGGATTG	45.0	50.0	57.8	58.0	868-916 bp	1.0	87.3
psbI-trnS-GCU_p4	TTTTCTTCGCCAAATTGCCC	GTGGACTAAAGCGTCGGATT	45.0	50.0	57.8	58.0	869-917 bp	1.0	87.3
psbI-trnS-GCU_p5	GTTTGGCAAGCTGCTGTAAG	ACAAAAGAAGTACTGGCCCG	50.0	50.0	57.9	58.1	997-1053 bp	0.666667	68.2