Hotspot

psbK-psbI

LSC · 7156-7581 · 426 bp

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Pi 0.0536

MarkerSeek score 81.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 32
pi: 0.0536
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACTACTGTGTAGACGAAGAGT	50.0	42.9	57.9	55.5	491-522 bp	1.0	60.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	ACTACTGTGTAGACGAAGAGTT	50.0	40.9	57.9	56.3	491-522 bp	1.0	58.9
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	ACTACTGTGTAGACGAAGAGT	45.0	42.9	58.3	55.5	488-519 bp	1.0	58.8
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	ACTACTGTGTAGACGAAGAGT	45.0	42.9	58.3	55.5	489-520 bp	1.0	58.8
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	ACTACTGTGTAGACGAAGAGT	45.0	42.9	58.3	55.5	490-521 bp	1.0	58.8