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rps16-trnQ-UUG

LSC · 6014-6519 · 506 bp

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Pi 0.0569

MarkerSeek score 81.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 41
pi: 0.0569
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	541-602 bp	1.0	88.0
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	552-613 bp	1.0	87.4
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	533-594 bp	1.0	82.9
rps16-trnQ-UUG_p4	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.2	58.1	553-614 bp	1.0	82.8
rps16-trnQ-UUG_p5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	540-601 bp	1.0	82.2