Hotspot

trnK-UUU-rps16

LSC · 4238-4829 · 592 bp

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Pi 0.092
MarkerSeek score 83.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
592
variable_sites
70
pi
0.092
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCAAAGACAACGAATTCCGG 50.0 50.0 58.2 58.0 1133-1255 bp 1.0 88.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCAAAGACAACGAATTCCGG 50.0 50.0 58.2 58.0 1132-1254 bp 1.0 88.6
trnK-UUU-rps16_p3 ATACCCCGGAGACAGATAGC GCAAAGACAACGAATTCCGG 55.0 50.0 58.1 58.0 1929 bp 0.333333 52.5
trnK-UUU-rps16_p4 ATACCCCGGAGACAGATAGC TCGATCCCGAAGAGAAGGAA 55.0 50.0 58.1 57.9 1531 bp 0.333333 51.6
trnK-UUU-rps16_p5 ATACCCCGGAGACAGATAGC GTGCAAAGCAAAGACAACGA 55.0 45.0 58.1 57.8 1936 bp 0.333333 51.2