Hotspot

rpl22

LSC · 85779-86165 · 387 bp

Back to Zephyranthes

Pi 0.00517

MarkerSeek score 37.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 387
variable_sites: 5
pi: 0.00517
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	CATTGGCGGGGGATAACCTA	47.6	55.0	58.1	59.2	493-495 bp	1.0	62.4
rpl22_p2	CCAAGTCTGAAACCAAGTGGA	ATTGGCGGGGGATAACCTA	47.6	52.6	58.1	57.7	492-494 bp	1.0	62.1
rpl22_p3	ACCAAGTCTGAAACCAAGTGG	CATTGGCGGGGGATAACCTA	47.6	55.0	58.3	59.2	494-496 bp	1.0	60.2
rpl22_p4	ACCAAGTCTGAAACCAAGTGG	ATTGGCGGGGGATAACCTA	47.6	52.6	58.3	57.7	493-495 bp	1.0	59.9
rpl22_p5	CCAAGTCTGAAACCAAGTGG	CATTGGCGGGGGATAACCTA	50.0	55.0	56.5	59.2	493-495 bp	1.0	59.4