Hotspot

atpB

LSC · 55045-56541 · 1497 bp

Back to Zephyranthes

Pi 0.00267

MarkerSeek score 58.80

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 9
pi: 0.00267
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CGTGGTTTGGTAATACGCCA	ACCAATCAACTTGCTATCGGA	50.0	42.9	58.6	57.4	1699-1704 bp	1.0	62.9
atpB_p2	CGTGGTTTGGTAATACGCCA	CCAATCAACTTGCTATCGGAC	50.0	47.6	58.6	57.0	1698-1703 bp	1.0	60.3
atpB_p3	GGGGCGTGGTTTGGTAATAC	ACCAATCAACTTGCTATCGGA	55.0	42.9	58.9	57.4	1703-1708 bp	1.0	60.2
atpB_p4	CGTGGTTTGGTAATACGCCA	AACCAATCAACTTGCTATCGGA	50.0	40.9	58.6	58.0	1700-1705 bp	1.0	59.6
atpB_p5	AGGGGCGTGGTTTGGTAATA	ACCAATCAACTTGCTATCGGA	50.0	42.9	59.0	57.4	1704-1709 bp	1.0	59.4