Hotspot

atpA

LSC · 10642-12165 · 1524 bp

Back to Zephyranthes

Pi 0.00525

MarkerSeek score 47.90

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 19
pi: 0.00525
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCTCGGTGGAAACCAAATT	AGCCTTACAGGGAGCTCTAG	45.0	55.0	58.0	57.9	2376-2382 bp	1.0	80.6
atpA_p2	AGCCTTCCAAGCTAACGATG	AGCCTTACAGGGAGCTCTAG	50.0	55.0	58.0	57.9	2080-2086 bp	1.0	80.5
atpA_p3	AGCTCGGTGGAAACCAAATT	AAAATTGAGCGTGAGAGCCA	45.0	45.0	58.0	58.1	2774-2780 bp	1.0	80.2
atpA_p4	AGCTCGGTGGAAACCAAATT	AAATTGAGCGTGAGAGCCAA	45.0	45.0	58.0	58.1	2773-2779 bp	1.0	80.2
atpA_p5	AGCTCGGTGGAAACCAAATT	AATTGAGCGTGAGAGCCAAA	45.0	45.0	58.0	58.1	2772-2778 bp	1.0	80.2