Hotspot

rpl32-trnL-UAG

SSC · 116316-117128 · 813 bp

Back to Zephyranthes

Pi 0.0133
MarkerSeek score 48.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
813
variable_sites
24
pi
0.0133
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAGTAAGA TTTAGAGTATGCCGCCACTC 40.0 50.0 55.6 57.4 913-930 bp 1.0 57.8
rpl32-trnL-UAG_p2 TGTGCGACAAACAAGTAAGAA TTTAGAGTATGCCGCCACTC 38.1 50.0 56.3 57.4 913-930 bp 1.0 56.0
rpl32-trnL-UAG_p3 TTGTGCGACAAACAAGTAAGA TTTAGAGTATGCCGCCACTC 38.1 50.0 56.3 57.4 914-931 bp 1.0 56.0
rpl32-trnL-UAG_p4 TGTGCGACAAACAAGTAAGA GCTCTAGCACTGCTTCCTAA 40.0 50.0 55.6 57.0 877-894 bp 1.0 54.9
rpl32-trnL-UAG_p5 TGTGCGACAAACAAGTAAGA TAGCACTGCTTCCTAAGAGC 40.0 50.0 55.6 57.0 873-890 bp 1.0 54.9