Hotspot

rpl22-rps19

IRb · 86166-86215 · 50 bp

Back to Zephyranthes

Pi 0.06

MarkerSeek score 75.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 50
variable_sites: 7
pi: 0.06
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GTTTCCTCATAAGAGCGCCC	TCTGACTTTCGCGAGACATG	55.0	50.0	58.7	58.0	211-218 bp	1.0	79.5
rpl22-rps19_p2	GTTTCCTCATAAGAGCGCCC	CCTACTCTGACTTTCGCGAG	55.0	55.0	58.7	57.8	216-223 bp	1.0	78.0
rpl22-rps19_p3	GTTTCCTCATAAGAGCGCCC	CTCTGACTTTCGCGAGACAT	55.0	50.0	58.7	57.7	212-219 bp	1.0	77.4
rpl22-rps19_p4	GCCCGCGAATCTGATCAATT	TCTGACTTTCGCGAGACATG	50.0	50.0	59.1	58.0	195-202 bp	1.0	77.0
rpl22-rps19_p5	CGTTTCCTCATAAGAGCGCC	TCTGACTTTCGCGAGACATG	55.0	50.0	59.1	58.0	212-219 bp	1.0	76.5