Hotspot

psbE-petL

LSC · 67208-68158 · 951 bp

Back to Zephyranthes

Pi 0.0102

MarkerSeek score 54.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 21
pi: 0.0102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1097-1115 bp	1.0	77.2
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1110-1128 bp	1.0	76.9
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCAGCCAATAGAAAACCGA	45.0	45.0	58.2	57.5	1106-1124 bp	1.0	76.2
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.8	57.5	1105-1123 bp	1.0	76.1
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	50.0	45.0	58.2	57.5	1099-1117 bp	1.0	75.7