Hotspot

trnT-UGU-trnL-UAA

LSC · 47669-48618 · 950 bp

Back to Zephyranthes

Pi 0.0142

MarkerSeek score 65.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 950
variable_sites: 29
pi: 0.0142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GGAAGTTCCCACTTAGCAGG	55.0	55.0	57.9	57.9	1060-1114 bp	1.0	81.4
trnT-UGU-trnL-UAA_p2	AGAGAAAAAGCCGGCTATCG	GGAAGTTCCCACTTAGCAGG	50.0	55.0	58.1	57.9	1111-1165 bp	0.8	73.2
trnT-UGU-trnL-UAA_p3	CGATAGAGAAAAAGCCGGCT	GGAAGTTCCCACTTAGCAGG	50.0	55.0	58.1	57.9	1115-1169 bp	0.8	73.2
trnT-UGU-trnL-UAA_p4	TCGATAGAGAAAAAGCCGGC	GGAAGTTCCCACTTAGCAGG	50.0	55.0	58.1	57.9	1116-1170 bp	0.8	73.2
trnT-UGU-trnL-UAA_p5	AGAGAAAAAGCCGGCTATCG	ATCCAATCAAGTCCGTAGCG	50.0	50.0	58.1	57.8	1073-1127 bp	0.8	72.4