Hotspot

petN-psbM

LSC · 29495-30463 · 969 bp

Back to Zephyranthes

Pi 0.00908

MarkerSeek score 57.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 19
pi: 0.00908
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	AAAACAGTCAGCCAAAATGA	45.0	35.0	58.0	53.9	1064-1079 bp	1.0	50.7
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAAATGA	55.0	35.0	58.1	53.9	1012-1027 bp	1.0	50.0
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	TGTAAAAACAGTCAGCCAAAATGA	45.0	33.3	58.0	57.8	1068-1083 bp	1.0	49.9
petN-psbM_p4	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAAATGA	55.0	35.0	58.1	53.9	1013-1028 bp	1.0	49.9
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	AAAAACAGTCAGCCAAAATGA	45.0	33.3	58.0	54.8	1065-1080 bp	1.0	49.7