Hotspot

psbA-trnK-UUU

LSC · 1153-1391 · 239 bp

Back to Zephyranthes

Pi 0.0151

MarkerSeek score 58.20

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 7
pi: 0.0151
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAAACACCGAACCATCCGAT	GTTTGGGGAGGGGTCTTTTT	45.0	50.0	57.8	57.9	436 bp	1.0	79.0
psbA-trnK-UUU_p2	TAAAGACGGTTTTCGGTGCT	GTTTGGGGAGGGGTCTTTTT	45.0	50.0	57.8	57.9	415 bp	1.0	78.6
psbA-trnK-UUU_p3	GCTTGTACTTTCGCGTCTCT	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	58.3	57.9	365 bp	1.0	78.4
psbA-trnK-UUU_p4	CACCGAACCATCCGATGTAA	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	57.7	57.9	432 bp	1.0	78.1
psbA-trnK-UUU_p5	GTAAAGACGGTTTTCGGTGC	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	57.7	57.9	416 bp	1.0	78.1