Hotspot

rpl16

LSC · 84421-85920 · 1500 bp

Back to Zelkova

Pi 0.00304

MarkerSeek score 63.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1500
variable_sites: 7
pi: 0.00304
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACTTCTTTCTTTTGGTTCTACA	TGACTTATCTTTAGACCTATTCGG	31.8	37.5	53.5	55.5	1585-1624 bp	1.0	42.7
rpl16_p2	AACTTCTTTCTTTTGGTTCTACA	TGACTTATCTTTAGACCTATTCGG	30.4	37.5	54.3	55.5	1586-1625 bp	1.0	42.7
rpl16_p3	GCAAAAGGCGAAGTAAAAA	TGACTTATCTTTAGACCTATTCGG	36.8	37.5	52.6	55.5	1662-1700 bp	1.0	42.6
rpl16_p4	GCAAAAGGCGAAGTAAAAA	TTGACTTATCTTTAGACCTATTCGG	36.8	36.0	52.6	56.2	1663-1701 bp	1.0	42.6
rpl16_p5	GCAAAAGGCGAAGTAAAAA	CTTGACTTATCTTTAGACCTATTCGG	36.8	38.5	52.6	57.2	1664-1702 bp	1.0	42.6