Hotspot

psbC

LSC · 35840-37261 · 1422 bp

Back to Zelkova

Pi 0.00293

MarkerSeek score 60.70

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 7
pi: 0.00293
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCTCAGGAAATCCGTGCAG	ACAACCTGACTCGTCTAACC	50.0	50.0	57.9	56.9	1625-1627 bp	1.0	70.8
psbC_p2	TTCTCAGGAAATCCGTGCAG	CAAGCATTGGATCTCCCGG	50.0	57.9	57.9	58.3	1552 bp	1.0	69.3
psbC_p3	GCAGCGGAAGATCCTGAATT	ACAACCTGACTCGTCTAACC	50.0	50.0	58.3	56.9	1609-1611 bp	1.0	69.0
psbC_p4	AGGGTATTCGTGCTTGGATG	ACAACCTGACTCGTCTAACC	50.0	50.0	57.7	56.9	1554-1556 bp	1.0	69.0
psbC_p5	TTCTCAGGAAATCCGTGCAG	CAACCTGACTCGTCTAACCT	50.0	50.0	57.9	56.6	1624-1626 bp	1.0	68.5