Hotspot

psbA

LSC · 273-1334 · 1062 bp

Back to Zelkova

Pi 0

MarkerSeek score 25.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AATCCACTTGGCTACATCCG	ATTCATGTGCTTGGGAGTCC	50.0	50.0	57.7	57.9	1337-1396 bp	1.0	77.8
psbA_p2	AATCCACTTGGCTACATCCG	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.7	57.9	1705-1765 bp	1.0	77.7
psbA_p3	AATCCACTTGGCTACATCCG	CGGCTTGGGGAGAGATTTTT	50.0	50.0	57.7	58.2	1675-1735 bp	1.0	77.5
psbA_p4	AATCCACTTGGCTACATCCG	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.7	57.8	1593-1653 bp	1.0	77.0
psbA_p5	AATCCACTTGGCTACATCCG	CTGTTGAATAACAAGCCCGC	50.0	50.0	57.7	57.7	1382-1441 bp	1.0	76.8