Hotspot

ndhA

SSC · 123800-126382 · 2583 bp

Back to Zelkova

Pi 0.0121

MarkerSeek score 52.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2583
variable_sites: 47
pi: 0.0121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGCAGCTCGTACTGTTTGTT	AAAATACGCCCACCAGGTTT	45.0	45.0	58.1	58.0	2831-2918 bp	1.0	83.1
ndhA_p2	TGCAGCTCGTACTGTTTGTT	AAATACGCCCACCAGGTTTT	45.0	45.0	58.1	58.0	2830-2917 bp	1.0	83.1
ndhA_p3	TTGCAGCTCGTACTGTTTGT	AAAATACGCCCACCAGGTTT	45.0	45.0	58.1	58.0	2832-2919 bp	1.0	83.1
ndhA_p4	TTGCAGCTCGTACTGTTTGT	AAATACGCCCACCAGGTTTT	45.0	45.0	58.1	58.0	2831-2918 bp	1.0	83.1
ndhA_p5	TGCAGCTCGTACTGTTTGTT	AGATGGAAAATACGCCCACC	45.0	50.0	58.1	57.9	2837-2924 bp	1.0	82.7