Hotspot

rps18-rpl20

LSC · 71369-71585 · 217 bp

Back to Zelkova

Pi 0.00614

MarkerSeek score 61.70

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 217
variable_sites: 2
pi: 0.00614
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	ATTTGAAAGAAGCGGGTCGA	TCTGTACAAGAAGCAGTTGC	45.0	45.0	57.8	56.0	364-381 bp	1.0	66.8
rps18-rpl20_p2	CGGGTCGACCACTAGAACTA	TCTGTACAAGAAGCAGTTGC	55.0	45.0	58.3	56.0	352-369 bp	1.0	66.4
rps18-rpl20_p3	ATTTGAAAGAAGCGGGTCGA	ATCTGTACAAGAAGCAGTTGC	45.0	42.9	57.8	56.5	365-382 bp	1.0	63.2
rps18-rpl20_p4	CGGGTCGACCACTAGAACTA	ATCTGTACAAGAAGCAGTTGC	55.0	42.9	58.3	56.5	353-370 bp	1.0	62.7
rps18-rpl20_p5	TTTGAAAGAAGCGGGTCGAC	TCTGTACAAGAAGCAGTTGC	50.0	45.0	58.8	56.0	363-380 bp	1.0	62.1