Hotspot

trnP-UGG-psaJ

LSC · 69699-70091 · 393 bp

Back to Zelkova

Pi 0.0017

MarkerSeek score 42.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 1
pi: 0.0017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	477-485 bp	1.0	80.1
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	476-484 bp	1.0	80.0
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	488-496 bp	1.0	80.0
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	487-495 bp	1.0	80.0
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	490-498 bp	1.0	80.0