Hotspot

trnW-CCA-trnP-UGG

LSC · 69473-69624 · 152 bp

Back to Zelkova

Pi 0.0526

MarkerSeek score 66.10

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 12
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	219-250 bp	1.0	83.0
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	221-252 bp	1.0	82.9
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	222-253 bp	1.0	82.9
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	232-263 bp	1.0	82.8
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	233-264 bp	1.0	82.8