Hotspot

accD-psaI

LSC · 62067-62544 · 478 bp

Back to Zelkova

Pi 0.00872

MarkerSeek score 65.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 6
pi: 0.00872
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGATCCAATTGTACCACGGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.5	58.0	631-650 bp	1.0	70.8
accD-psaI_p2	TGATCCAATTGTACCACGGA	AGAAACCATTGCAATTGCCG	45.0	45.0	56.5	57.9	636-655 bp	1.0	70.3
accD-psaI_p3	GCTCCATGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	577-596 bp	1.0	69.0
accD-psaI_p4	TGATCCAATTGTACCACGGAA	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.3	58.0	631-650 bp	1.0	68.8
accD-psaI_p5	TTGATCCAATTGTACCACGGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.3	58.0	632-651 bp	1.0	68.8