Hotspot

ndhC-trnV-UAC

LSC · 53808-54561 · 754 bp

Back to Zelkova

Pi 0.012

MarkerSeek score 63.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 13
pi: 0.012
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	873-923 bp	1.0	82.5
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	872-922 bp	1.0	79.7
ndhC-trnV-UAC_p3	TAATCGGGGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	878-928 bp	1.0	78.7
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	861-911 bp	1.0	78.6
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	TGGGTCTTTGAAACAGCTCA	50.0	45.0	57.9	57.3	931-981 bp	1.0	78.2