Hotspot

psbZ-trnG-GCC

LSC · 38158-38588 · 431 bp

Back to Zelkova

Pi 0.0186
MarkerSeek score 68.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
431
variable_sites
13
pi
0.0186
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG AATCGAACCCGCATCTTCTC 55.0 50.0 58.0 58.1 595-607 bp 1.0 82.2
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG GAATCGAACCCGCATCTTCT 55.0 50.0 58.0 58.1 596-608 bp 1.0 82.2
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 598-610 bp 1.0 80.7
psbZ-trnG-GCC_p4 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 599-611 bp 1.0 80.7
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 596-608 bp 1.0 80.3