Hotspot

rpoB-trnC-GCA

LSC · 28000-29345 · 1346 bp

Back to Zelkova

Pi 0.00616

MarkerSeek score 64.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 13
pi: 0.00616
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTTCCTCATTTCCACCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.4	58.1	1430-1468 bp	1.0	77.2
rpoB-trnC-GCA_p2	GCTCTTCTGTTAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.2	58.1	1507-1545 bp	1.0	75.3
rpoB-trnC-GCA_p3	AGCTCTTCTGTTAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.2	58.1	1508-1546 bp	1.0	75.2
rpoB-trnC-GCA_p4	ATTTCCTCATTTCCACCCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.4	58.4	1412-1450 bp	1.0	75.0
rpoB-trnC-GCA_p5	TTCCTCATTTCCACCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.8	58.1	1428-1466 bp	1.0	74.9