Hotspot

trnG-GCC-trnR-UCU

LSC · 10537-10908 · 372 bp

Back to Zelkova

Pi 0.0193

MarkerSeek score 61.40

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 11
pi: 0.0193
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	380-485 bp	1.0	69.4
trnG-GCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	383-488 bp	1.0	69.1
trnG-GCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	386-491 bp	1.0	68.7
trnG-GCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	389-494 bp	1.0	68.4
trnG-GCC-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	384-489 bp	1.0	66.9