Hotspot

trnS-GCU-trnG-GCC

LSC · 8927-9775 · 849 bp

Back to Zelkova

Pi 0.025

MarkerSeek score 74.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 34
pi: 0.025
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TAATCAAACCGAGGGATCCC	50.0	50.0	58.0	56.7	871-1051 bp	1.0	76.2
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TAATCAAACCGAGGGATCCC	50.0	50.0	58.0	56.7	870-1050 bp	1.0	76.2
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	TAATCAAACCGAGGGATCCC	50.0	50.0	58.2	56.7	925-1105 bp	1.0	74.9
trnS-GCU-trnG-GCC_p4	CAATCCGACGCTTTAGTCCA	TTAATCAAACCGAGGGATCCC	50.0	47.6	58.0	57.4	872-1052 bp	1.0	74.2
trnS-GCU-trnG-GCC_p5	AATCCGACGCTTTAGTCCAC	TTAATCAAACCGAGGGATCCC	50.0	47.6	58.0	57.4	871-1051 bp	1.0	74.2