Hotspot

matK-rps16

LSC · 3467-5291 · 1825 bp

Back to Zelkova

Pi 0.0108

MarkerSeek score 55.90

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1825
variable_sites: 31
pi: 0.0108
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CCACGATCATGAGCAAATGC	GCCGTACGAGGAGAAAACTT	50.0	50.0	57.9	57.9	2199-2255 bp	1.0	82.5
matK-rps16_p2	TGCAACGAAGAGGCATCTTT	GCCGTACGAGGAGAAAACTT	45.0	50.0	58.1	57.9	2613-2669 bp	1.0	82.2
matK-rps16_p3	AATGCAACGAAGAGGCATCT	GCCGTACGAGGAGAAAACTT	45.0	50.0	57.9	57.9	2615-2671 bp	1.0	82.0
matK-rps16_p4	CCACGATCATGAGCAAATGC	TCGATCCCGAAGAGAAGGAA	50.0	50.0	57.9	57.9	2098-2157 bp	1.0	82.0
matK-rps16_p5	TGCAACGAAGAGGCATCTTT	TCGATCCCGAAGAGAAGGAA	45.0	50.0	58.1	57.9	2512-2571 bp	1.0	81.7