Hotspot

ycf1

SSC · 126776-132286 · 5511 bp

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Pi 0.0107

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5511
variable_sites: 152
pi: 0.0107
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAAGGTCCGCTCAACAACG	TGGAATTATCTCAATGAACAAAA	52.6	26.1	57.8	52.6	188 bp	1.0	44.3
ycf1_p2	CGATAAGGTCCGCTCAACAA	TGGAATTATCTCAATGAACAAAA	50.0	26.1	58.0	52.6	191 bp	1.0	44.2
ycf1_p3	CGATAAGGTCCGCTCAACAA	TGGAATTATCTCAATGAACAAAAA	50.0	25.0	58.0	53.4	191 bp	1.0	44.2
ycf1_p4	ATAAGGTCCGCTCAACAACG	TGGAATTATCTCAATGAACAAAA	50.0	26.1	58.3	52.6	189 bp	1.0	44.2
ycf1_p5	ATAAGGTCCGCTCAACAACG	TGGAATTATCTCAATGAACAAAAA	50.0	25.0	58.3	53.4	189 bp	1.0	44.2