Hotspot

psaB

LSC · 39671-41875 · 2205 bp

Back to Zehneria

Pi 0.00238

MarkerSeek score 59.10

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 13
pi: 0.00238
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	AAGATCCCCTTCTCCATCGA	ACAACATGGGCGTTCTTCTT	50.0	45.0	57.5	58.0	2332 bp	1.0	76.5
psaB_p2	AGATCCCCTTCTCCATCGAA	ACAACATGGGCGTTCTTCTT	50.0	45.0	57.5	58.0	2331 bp	1.0	76.5
psaB_p3	AAGATCCCCTTCTCCATCGA	GCAGTCGGATAATGGCTAGG	50.0	55.0	57.5	57.9	2299 bp	1.0	75.9
psaB_p4	AGATCCCCTTCTCCATCGAA	GCAGTCGGATAATGGCTAGG	50.0	55.0	57.5	57.9	2298 bp	1.0	75.9
psaB_p5	AGATCCCCTTCTCCATCGAA	GGGCGTTCTTCTTAGCAAGA	50.0	50.0	57.5	57.9	2324 bp	1.0	75.9