Hotspot

rps15-ycf1

SSC · 126375-126775 · 401 bp

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Pi 0.0213

MarkerSeek score 57.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 21
pi: 0.0213
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ATGAGTCGAAGAACACCGTG	TCCCCTAGAGGTCGTAGAGA	50.0	55.0	57.9	57.8	1164-1176 bp	1.0	80.4
rps15-ycf1_p2	CATTGATGGGTGGTGAGGTC	TCCCCTAGAGGTCGTAGAGA	55.0	55.0	58.2	57.8	1144-1156 bp	1.0	78.9
rps15-ycf1_p3	CCCAACTGTTCTGGACCATT	TCCCCTAGAGGTCGTAGAGA	50.0	55.0	57.7	57.8	1347-1359 bp	1.0	78.4
rps15-ycf1_p4	TGCGAAATCACTGAGCTGAA	TCCCCTAGAGGTCGTAGAGA	45.0	55.0	57.8	57.8	742-743 bp	0.714286	67.6
rps15-ycf1_p5	TTGCGAAATCACTGAGCTGA	TCCCCTAGAGGTCGTAGAGA	45.0	55.0	57.8	57.8	743-744 bp	0.714286	67.6