Hotspot

rpl32-trnL

SSC · 116183-117040 · 858 bp

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Pi 0.0174

MarkerSeek score 60.60

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 858
variable_sites: 39
pi: 0.0174
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TCAACGGGGAATTCACAAAGT	AGCGTGTCTACCGATTTCAC	42.9	50.0	58.1	58.0	922-943 bp	1.0	75.3
rpl32-trnL_p2	TCAACGGGGAATTCACAAAGT	CAGCGTGTCTACCGATTTCA	42.9	50.0	58.1	58.0	923-944 bp	1.0	75.3
rpl32-trnL_p3	TCAACGGGGAATTCACAAAGT	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	58.1	57.9	932-953 bp	1.0	74.3
rpl32-trnL_p4	TCAACGGGGAATTCACAAAGT	CCTAAGAGCAGCGTGTCTAC	42.9	55.0	58.1	57.8	931-952 bp	1.0	73.8
rpl32-trnL_p5	TCAACGGGGAATTCACAAAGT	CTAAGAGCAGCGTGTCTACC	42.9	55.0	58.1	57.8	930-951 bp	1.0	73.8