Hotspot

petA-psbJ

LSC · 65422-66420 · 999 bp

Back to Zehneria

Pi 0.0129
MarkerSeek score 59.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
999
variable_sites
31
pi
0.0129
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAGAAGGTTCAATTGTCCGA CCTGTGATCGGTTTAGTCGG 42.9 55.0 57.2 58.1 1060-1100 bp 1.0 71.2
petA-psbJ_p2 TGAGAAGGTTCAATTGTCCGA TCCGGCTTAGGTTCATCTCT 42.9 50.0 57.2 57.8 1018-1058 bp 1.0 70.7
petA-psbJ_p3 TGAGAAGGTTCAATTGTCCGA CCGGCTTAGGTTCATCTCTG 42.9 55.0 57.2 57.8 1017-1057 bp 1.0 70.2
petA-psbJ_p4 TGAGAAGGTTCAATTGTCCGAA CCTGTGATCGGTTTAGTCGG 40.9 55.0 57.9 58.1 1060-1100 bp 1.0 68.6
petA-psbJ_p5 TTGAGAAGGTTCAATTGTCCGA CCTGTGATCGGTTTAGTCGG 40.9 55.0 57.9 58.1 1061-1101 bp 1.0 68.6