Hotspot

rpoB-trnC

LSC · 27927-29081 · 1155 bp

Back to Zehneria

Pi 0.0138

MarkerSeek score 50.00

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 38
pi: 0.0138
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	GATACCGGGGCTTTGCAATA	TGTTTGTTGATGAGGCGACA	50.0	45.0	58.0	58.0	1635-1638 bp	1.0	82.0
rpoB-trnC_p2	CGAGCCCATATCCTTGCTTT	TGTTTGTTGATGAGGCGACA	50.0	45.0	58.0	58.0	1751-1754 bp	1.0	81.9
rpoB-trnC_p3	CCGGGGCTTTGCAATATTTG	TGTTTGTTGATGAGGCGACA	50.0	45.0	58.1	58.0	1631-1634 bp	1.0	81.7
rpoB-trnC_p4	GATACCGGGGCTTTGCAATA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1614-1617 bp	1.0	81.7
rpoB-trnC_p5	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1730-1733 bp	1.0	81.6