Hotspot

trnK-rps16

LSC · 4198-5128 · 931 bp

Back to Zehneria

Pi 0.014

MarkerSeek score 62.80

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 931
variable_sites: 31
pi: 0.014
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	CGCACTTAAAAGCCGAGTAC	GTGCTCAACCAACAGGAACT	50.0	50.0	57.2	58.3	1010-1093 bp	1.0	76.7
trnK-rps16_p2	CGCACTTAAAAGCCGAGTAC	AGGCAGGTGTTTTTACGGAA	50.0	45.0	57.2	57.6	971-1054 bp	1.0	76.7
trnK-rps16_p3	CGCACTTAAAAGCCGAGTAC	AAGGCAGGTGTTTTTACGGA	50.0	45.0	57.2	57.6	972-1055 bp	1.0	76.6
trnK-rps16_p4	CGCACTTAAAAGCCGAGTAC	GGCAGGTGTTTTTACGGAAC	50.0	50.0	57.2	57.6	970-1053 bp	1.0	76.2
trnK-rps16_p5	AGCCGAGTACTCTACCATTGA	GTGCTCAACCAACAGGAACT	47.6	50.0	58.0	58.3	1000-1083 bp	1.0	74.9