Hotspot

trnL-CAA-trnI-CAU

IRa · 134511-138108 · 3598 bp

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Pi 0.000238
MarkerSeek score 47.00
Primer available yes
Label trnL-CAA-trnI-CAU

Feature Metadata

label_name
trnL-CAA-trnI-CAU
feature_type
igs
region
IRa
length_bp
3598
variable_sites
3
pi
0.000238
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-trnI-CAU_p1 AAATGGTAGACACGCGAGAC ACCACCCTTGCAGTAGGATA 50.0 50.0 58.0 57.7 80-289 bp 1.0 78.1
trnL-CAA-trnI-CAU_p2 TGAAATGGTAGACACGCGAG ACCACCCTTGCAGTAGGATA 50.0 50.0 58.0 57.7 80-291 bp 1.0 78.1
trnL-CAA-trnI-CAU_p3 TGGTGAAATGGTAGACACGC ACCACCCTTGCAGTAGGATA 50.0 50.0 58.2 57.7 80-294 bp 1.0 76.6
trnL-CAA-trnI-CAU_p4 AAATGGTAGACACGCGAGAC CCTTGCAGTAGGATACCGTC 50.0 55.0 58.0 57.5 80-284 bp 1.0 76.2
trnL-CAA-trnI-CAU_p5 TGAAATGGTAGACACGCGAG CCTTGCAGTAGGATACCGTC 50.0 55.0 58.0 57.5 80-286 bp 1.0 76.2