Hotspot

trnI-CAU-trnL-CAA

IRb · 85302-88899 · 3598 bp

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Pi 0.000238
MarkerSeek score 47.00
Primer available yes
Label trnI-CAU-trnL-CAA

Feature Metadata

label_name
trnI-CAU-trnL-CAA
feature_type
igs
region
IRb
length_bp
3598
variable_sites
3
pi
0.000238
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-trnL-CAA_p1 CCACAAGAACGAAAGCACCT GGTCTTATCTTGGCTCGCAA 50.0 50.0 58.7 58.0 80-242 bp 1.0 74.6
trnI-CAU-trnL-CAA_p2 CGAGGGCTTCTTATTCCACA GGTCTTATCTTGGCTCGCAA 50.0 50.0 57.3 58.0 80-257 bp 1.0 74.6
trnI-CAU-trnL-CAA_p3 ACGAGGGCTTCTTATTCCAC GGTCTTATCTTGGCTCGCAA 50.0 50.0 57.3 58.0 80-258 bp 1.0 74.6
trnI-CAU-trnL-CAA_p4 TCCACAAGAACGAAAGCACC GGTCTTATCTTGGCTCGCAA 50.0 50.0 58.7 58.0 80-243 bp 1.0 74.6
trnI-CAU-trnL-CAA_p5 CCACAAGAACGAAAGCACCT GAAAAGGGTCCCATGATCCC 50.0 55.0 58.7 57.9 80-267 bp 1.0 74.2