Hotspot

rpl33-rps18

LSC · 67512-67854 · 343 bp

Pi 0.0051

MarkerSeek score 56.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 5
pi: 0.0051
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AAAGGAGGTTCATGGCCAAG	TCGAGAACGCCTACGAAAAG	50.0	50.0	58.1	58.0	80-720 bp	1.0	83.9
rpl33-rps18_p2	GAAAGGAGGTTCATGGCCAA	TCGAGAACGCCTACGAAAAG	50.0	50.0	58.1	58.0	80-721 bp	1.0	83.8
rpl33-rps18_p3	AAAGGAGGTTCATGGCCAAG	AACGCCTACGAAAAGGTTGT	50.0	45.0	58.1	58.0	80-715 bp	1.0	83.7
rpl33-rps18_p4	AAAGGAGGTTCATGGCCAAG	ACGCCTACGAAAAGGTTGTT	50.0	45.0	58.1	58.0	80-714 bp	1.0	83.7
rpl33-rps18_p5	GAAAGGAGGTTCATGGCCAA	ACGCCTACGAAAAGGTTGTT	50.0	45.0	58.1	58.0	80-715 bp	1.0	83.7