Hotspot

rbcL-psaI

LSC · 58747-59636 · 890 bp

Pi 0.000642

MarkerSeek score 46.00

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 2
pi: 0.000642
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.0	57.9	819-1076 bp	1.0	79.7
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.2	57.9	798-1055 bp	1.0	78.4
rbcL-psaI_p3	TGCAAATGGAGTGCTGAACT	GAAGTCATTGCAATTGCCGG	45.0	50.0	58.0	58.4	819-1079 bp	1.0	77.3
rbcL-psaI_p4	TGCAAATGGAGTGCTGAACT	GGAAGTCATTGCAATTGCCG	45.0	50.0	58.0	58.4	819-1080 bp	1.0	77.3
rbcL-psaI_p5	GCCGCAGCTTGTGAAATATG	GAAGTCATTGCAATTGCCGG	50.0	50.0	58.2	58.4	798-1058 bp	1.0	76.1