Hotspot

psbM-petN

LSC · 18752-19547 · 796 bp

Pi 0.00542

MarkerSeek score 37.90

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 12
pi: 0.00542
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGTTCATTCTAGTTCCTACTGCC	GTAGTATGGGGGAGGAGTGG	43.5	60.0	57.9	58.3	891-896 bp	1.0	54.5
psbM-petN_p2	TGTTCATTCTAGTTCCTACTGCC	TAGTATGGGGGAGGAGTGGA	43.5	55.0	57.9	58.4	890-895 bp	1.0	53.8
psbM-petN_p3	TGTTCATTCTAGTTCCTACTGCC	CGCTAGTAGTATGGGGGAGG	43.5	60.0	57.9	58.5	896-901 bp	1.0	53.1
psbM-petN_p4	GTTCATTCTAGTTCCTACTGCCT	GTAGTATGGGGGAGGAGTGG	43.5	60.0	57.7	58.3	890-895 bp	1.0	52.4
psbM-petN_p5	TGTTCATTCTAGTTCCTACTGCC	TCGCTAGTAGTATGGGGGAG	43.5	55.0	57.9	57.4	897-902 bp	1.0	52.1