Hotspot

trnY-GUA-trnD-GUC

LSC · 17169-17516 · 348 bp

Back to Zea

Pi 0.00411
MarkerSeek score 35.80
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
348
variable_sites
3
pi
0.00411
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 AATTCTTCCTGGGTCGATGC CGGTGCTCTGACCAATTGAA 50.0 50.0 58.0 58.5 472 bp 1.0 76.3
trnY-GUA-trnD-GUC_p2 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.4 58.5 439 bp 1.0 73.9
trnY-GUA-trnD-GUC_p3 GGGACGGACTGTAAATTCGT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.6 58.5 438 bp 1.0 73.7
trnY-GUA-trnD-GUC_p4 AATTCTTCCTGGGTCGATGC GGTGCTCTGACCAATTGAAC 50.0 50.0 58.0 57.0 471 bp 1.0 72.2
trnY-GUA-trnD-GUC_p5 TGGGGACGGACTGTAAATTC CGGTGCTCTGACCAATTGAA 50.0 50.0 57.2 58.5 440 bp 1.0 70.5