Hotspot

rps16-trnQ-UUG

LSC · 5599-7041 · 1443 bp

Back to Zea

Pi 0.00356
MarkerSeek score 53.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1443
variable_sites
14
pi
0.00356
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.81
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 45.0 50.0 58.1 57.6 1247-1523 bp 0.857143 74.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA ACTCGGAGGTTCGAATCCTT 45.0 50.0 58.1 58.4 1246-1522 bp 0.857143 74.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGTTACTC 45.0 55.0 58.1 58.5 1262-1538 bp 0.857143 74.2
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC TACTCGGAGGTTCGAATCCT 50.0 50.0 58.2 57.6 1258-1534 bp 0.857143 74.0
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC ACTCGGAGGTTCGAATCCTT 50.0 50.0 58.2 58.4 1257-1533 bp 0.857143 73.9