Hotspot

ccsA-ndhD

SSC · 124081-124668 · 588 bp

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Pi 0.133
MarkerSeek score 84.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
588
variable_sites
77
pi
0.133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGGATCAATCTATTAGGAATCGG CAGTTGACAAGGTCGAAGCT 41.7 50.0 57.7 58.1 687-783 bp 1.0 53.8
ccsA-ndhD_p2 TGGGATCAATCTATTAGGAATCGG TCAGTTGACAAGGTCGAAGC 41.7 50.0 57.7 58.1 688-784 bp 1.0 53.7
ccsA-ndhD_p3 GGAATCGGACTACATAGTTATGGT CAGTTGACAAGGTCGAAGCT 41.7 50.0 57.7 58.1 671-767 bp 1.0 53.5
ccsA-ndhD_p4 GGAATCGGACTACATAGTTATGGT TCAGTTGACAAGGTCGAAGC 41.7 50.0 57.7 58.1 672-768 bp 1.0 53.4
ccsA-ndhD_p5 TCGGACTACATAGTTATGGTTCA CAGTTGACAAGGTCGAAGCT 39.1 50.0 56.6 58.1 667-763 bp 1.0 52.7